Introduction the virapower lentiviral expression system allows creation of a replication incompetent, hiv1based lentivirus that is used to deliver and express your gene of interest in either dividing or nondividing mammalian cells. Hat protein expression and purification system user manual. The virapower lentiviral expression system allows creation of a replication incompetent, hiv1based lentivirus that is used to deliver and express your gene of interest in either dividing or nondividing mammalian cells. Lentix lentiviral expression systems user manual 062619 takara bio usa, inc. Lentix teton advanced inducible expression system user.
A central polypurine tractcentral termination sequence creates a dna flap that increases nuclear importation of the viral genome during target cell infection. Clontechlentix lentiviral expression systems user manual protocol no. Lentix teton advanced inducible expression system user manual a. Lentiviruses can be used for either transient or stable expression, and they can infect both replicative and quiescent cells.
Lentix htx is a 4th generation lentiviral packaging system that delivers the gag and pol genes on separate packaging vectors figure 3, which precludes the formation. Lentix idimerize inducible homodimer system with teton 3g user manual 011018 takarabio. After cotransducing your target cells with your lvxtet3g and lvxtre3ghom1goi lentiviral stocks, you are ready to study the effects of dimerization in your cells of interest. Lentix tetone inducible expression system user manual 011118 takara bio usa, inc. Summary thelentix teton advanced inducible expression system uses a highly efficient, lentiviralbased gene transfer technology to deliver our tightly regulated and highly responsive teton advanced gene expression system into. Lentix crisprcas9 system user manual 1216 takarabio. Lentix idimerize inducible homodimer system with tet. Getting the most from any lentiviral packaging system requires a host 293t cell line that transfects easily and supports highlevel expression of viral proteins.